Publications

Authors:

Busato, Alice; Bonafede, Roberta; Bontempi, Pietro; Scambi, Ilaria; Schiaffino, Lorenzo; Benati, Donatella; Malatesta, Manuela; Sbarbati, Andrea; Marzola, Pasquina; Mariotti, Raffaella

Title:

Labeling and magnetic resonance imaging of exosomes isolated from adipose stem cells

Year:

2017

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

CURRENT PROTOCOLS IN CELL BIOLOGY

ISSN of journal:

1934-2500

N° Volume:

75

Page numbers:

3.44.1-3.44.15

Keyword:

MRI; exosome labeling; stem cells labeling; superparamagnetic iron oxide nanoparticles

Short description of contents:

Adipose stem cells (ASC) represent a promising therapeutic approach for neurodegenerative diseases. Most biological effects of ASC are probably mediated by extracellular vesicles, such as exosomes, which influence the surrounding cells. Current development of exosome therapies requires efficient and noninvasive methods to localize, monitor, and track the exosomes. Among imaging methods used for this purpose, magnetic resonance imaging (MRI) has advantages: high spatial resolution, rapid in vivo acquisition, and radiation-free operation. To be detectable with MRI, exosomes must be labeled with MR contrast agents, such as ultra-small superparamagnetic iron oxide nanoparticles (USPIO). Here, we set up an innovative approach for exosome labeling that preserves their morphology and physiological characteristics. We show that by labeling ASC with USPIO before extraction of nanovesicles, the isolated exosomes retain nanoparticles and can be visualized by MRI. The current work aims at validating this novel USPIO-based exosome labeling method by monitoring the efficiency of the labeling with MRI both in ASC and in exosomes. © 2017 by John Wiley & Sons, Inc.

Web page:

https://doi.org/10.1002/cpcb.23

Product ID:

98267

Handle IRIS:

11562/965714

Last Modified:

November 13, 2022

Bibliographic citation:

Busato, Alice; Bonafede, Roberta; Bontempi, Pietro; Scambi, Ilaria; Schiaffino, Lorenzo; Benati, Donatella; Malatesta, Manuela; Sbarbati, Andrea; Marzola, Pasquina; Mariotti, Raffaella, Labeling and magnetic resonance imaging of exosomes isolated from adipose stem cells «CURRENT PROTOCOLS IN CELL BIOLOGY» , vol. 75 , 2017 , pp. 3.44.1-3.44.15

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