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The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation.  (2010)

Authors:
Valenti MT; Giannini S; Donatelli L; Zanatta M; Bertoldo F; Sella S; Vilei MT; Ossi E; Realdi G; Lo Cascio V; Dalle Carbonare L.
Title:
The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation.
Year:
2010
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Format:
Elettronico
Referee:
Name of journal:
ARTHRITIS RESEARCH & THERAPY
ISSN of journal:
1478-6362
N° Volume:
12
Number or Folder:
4
Page numbers:
R163-R176
Keyword:
COX-2; glucocorticoids; histomorphometry; osteoporosis; risedronate
Short description of contents:
Introduction: The purpose of this study was to evaluate the effects of risedronate (Ris) in the modulation of bone formation in rats with glucocorticoid (GC)-induced osteoporosis by histomorphometric, immunohistochemical and gene expression analyses. Methods: We analyzed structure, turnover and microarchitecture, cyclooxygenase 2 (COX-2) levels and osteocyte apoptosis in 40 female rats divided as follows: 1) vehicle of methylprednisolone (vGC) + vehicle of risedronate (vRis); 2) Ris 5 μg/Kg + vGC; 3) methylprednisolone (GC) 7 mg/Kg + vRis; 4) GC 7 mg/Kg +Ris 5 μg/Kg. In addition, we evaluated cell proliferation and expression of COX-2 and bone alkaline phosphatase (b-ALP) genes in bone marrow cells and MLO-y4 osteocytes treated with Ris alone or in co-treatment with the selective COX-2 inhibitor NS-398 or with dexametasone. Results: Ris reduced apoptosis induced by GC of osteocytes (41% vs 86%, P < 0.0001) and increased COX-2 expression with respect to controls (Immuno-Hystochemical Score (IHS): 8.75 vs 1.00, P < 0.0001). These positive effects of Ris in bone formation were confirmed by in vitro data as the viability and expression of b-ALP gene in bone marrow cells resulted increased in a dose dependent manner. Conclusions: These findings suggest a positive effect of Ris in bone formation and support the hypothesis that the up-regulation of COX-2 could be an additional mechanism of anabolic effect of Ris.
Product ID:
58066
Handle IRIS:
11562/346048
Deposited On:
February 29, 2012
Last Modified:
August 16, 2021
Attachments:
1
Bibliographic citation:
Valenti MT; Giannini S; Donatelli L; Zanatta M; Bertoldo F; Sella S; Vilei MT; Ossi E; Realdi G; Lo Cascio V; Dalle Carbonare L., The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation. «ARTHRITIS RESEARCH & THERAPY» , vol. 12 , n. 42010pp. R163-R176

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Effetto del risedronato nell'osteoporosi cortisonica su modello animale Department Medicina Luca Giuseppe Dalle Carbonare
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