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Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis.  (2009)

Authors:
Dalle Carbonare L; Valenti MT; Zanatta M; Donatelli L; Lo Cascio V.
Title:
Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis.
Year:
2009
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Format:
A Stampa
Referee:
Name of journal:
Arthritis and Rheumatism
ISSN of journal:
0004-3591
N° Volume:
60
Number or Folder:
11
Page numbers:
3356-3365
Keyword:
mesenchymal stem cells; osteoporosis; osteoblastic lineage
Short description of contents:
Objective. While the role of osteoclasts in bone loss has been well investigated, the involvement of osteoblast-lineage cells has not been completely elucidated. Several genes contribute to normal osteoblastic differentiation from mesenchymal stem cells (MSCs), but an understanding of their role in the pathogenesis of osteoporosis is still lacking. The present study was undertaken to evaluate a possible alteration of osteogenic gene expression as a mechanism contributing to bone loss. Methods. We studied the osteogenic differentiation process in MSCs obtained from the peripheral blood of 31 patients with osteoporosis and 20 normal donors. The cells were evaluated by colony-forming unit–fibroblastic assay and cultured in osteogenic medium to analyze the transcription factors runt-related transcription factor 2 (RUNX-2) and Sp7 and the bone-related genes COL1A1, SPARC, and SPP1 after 3, 8, and 15 days of differentiation. In addition, to determine possible differences between the 2 groups in terms of osteoclastic and osteoblastic activation, we quantified the osteoprotegerin (OPG) and RANKL levels in the supernatants of osteoblastic culture. Results. CirculatingMSCswere increased in osteoporosis patients compared with normal donors. In contrast, gene expression analysis revealed downregulation of RUNX2, Sp7, COL1A1, SPARC, and SPP1 in patients with osteoporosis, associated with a lower OPG:RANKL ratio. Conclusion. These results suggest that an alteration of osteoblastic differentiation may contribute to the pathogenesis of osteoporosis. The noninvasive approach used in the present study could be proposed as a useful tool for studying mesenchymal involvement in bone diseases.
Product ID:
52981
Handle IRIS:
11562/338048
Deposited On:
February 29, 2012
Last Modified:
October 28, 2021
Bibliographic citation:
Dalle Carbonare L; Valenti MT; Zanatta M; Donatelli L; Lo Cascio V., Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis. «Arthritis and Rheumatism» , vol. 60 , n. 112009pp. 3356-3365

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