Pubblicazioni

Autori:

Valenti, Maria Teresa; Azzarello, G; Vinante, O; Manconi, R; Balducci, E; Guidolin, D; Chiavegato, A; Sartore, S.

Titolo:

Differentiation, proliferation and apoptosis levels in human leiomyoma and leiomyosarcoma

Anno:

1998

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Journal of Cancer Research and Clinical Oncology

ISSN Rivista:

0171-5216

N° Volume:

124

Numero o Fascicolo:

2

Intervallo pagine:

93-105

Parole chiave:

Leiomyoma; Leiomyosarcoma; Myosin isoforms; Smooth muscle cells;

Breve descrizione dei contenuti:

A comparative analysis of the differentiation pattern, the proliferative behaviour, and the level of apoptosis between human benign and malignant neoplasms of smooth-muscle (SM) tissue is lacking. The clinical, histopathological, immunochemical, and immunocytochemical features of leiomyomas (LM) and leiomyosarcomas (LMS) were investigated by a panel of monoclonal antibodies specific for some differentiation markers of SM tissue (SM myosin and alpha-actin, desmin, and SM22) and for markers of non-muscle tissue (vimentin and non-muscle myosin). Proliferating normal and neoplastic cells were identified by proliferating-cell nuclear antigen (PCNA)/Ki67 immunostainings and the apoptotic cells were revealed by means of the terminal-deoxynucleotidyltransferase-mediated dUTP nick-end labelling technique. Gel electrophoresis and Western blotting, performed with anti-(SM1/SM2 myosin isoform) antibody, indicated quantitative differences between LMS and LM, which mirrored higher positive to negative nuclear ratios for PCNA, Ki67 and apoptosis in malignant as opposed to benign neoplasms. With LM, however, a similar SM1 to SM2 ratio could be associated with different proliferation levels. Uterine, gastric and intestinal LMS displayed specific patterns of SM1/SM2 and/or non-muscle myosin expression that were not paralleled by different levels of proliferation/apoptosis. While the level of PCNA/Ki67 correlated with the level of apoptosis in normal SM tissues and LM, that of LMS did not. In vivo at the cellular level, LM and uterine LMS displayed a near-uniform SM tissue differentiation, whereas the other LMS displayed a lesser or a heterogeneous immunoreactivity. In vitro, cultured LMS cells showed a limited and peculiar expression of SM myosin. In conclusion, there is no reciprocal relationship between degree of differentiation and the level of proliferation, as exemplified by the finding that the less differentiated intestinal LMS displays the lowest proliferative behaviour and that the relatively more differentiated gastric LMS/metastasis is more proliferative.

Id prodotto:

50755

Handle IRIS:

11562/333490

depositato il:

29 ottobre 2013

ultima modifica:

1 dicembre 2023

Citazione bibliografica:

Valenti, Maria Teresa; Azzarello, G; Vinante, O; Manconi, R; Balducci, E; Guidolin, D; Chiavegato, A; Sartore, S., Differentiation, proliferation and apoptosis levels in human leiomyoma and leiomyosarcoma «Journal of Cancer Research and Clinical Oncology» , vol. 124 , n. 2 , 1998 , pp. 93-105

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