Molecular biology workshop (2016/2017)

Course code
Stefano Capaldi
Academic sector
Language of instruction
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
Teoria 2 I sem. Stefano Capaldi
Laboratorio [II turno] 4 I sem. Maria Teresa Valenti
Laboratorio [I turno] 4 I sem. Stefano Capaldi

Lesson timetable

I sem.
Activity Day Time Type Place Note
Teoria Thursday 1:30 PM - 3:30 PM lesson Lecture Hall B  
Laboratorio [I turno] Friday 9:30 AM - 1:30 PM laboratorio Laboratory Laboratorio di Biotecnologie genetiche from Oct 28, 2016  to Nov 11, 2016
Laboratorio [I turno] Friday 2:30 PM - 6:30 PM laboratorio Laboratory Laboratorio di Biotecnologie genetiche from Oct 28, 2016  to Nov 11, 2016
Laboratorio [II turno] Friday 10:30 AM - 6:30 PM laboratorio Laboratory Laboratorio di Biotecnologie genetiche from Nov 18, 2016  to Jan 31, 2017

Learning outcomes

The primary educational aim of the course is to provide the student an overview on the most common methodologies and techniques for the manipulation of DNA. In particular, the course covers the main techniques for purification of nucleic acids, their separation by electrophoresis, DNA amplification and methods for cloning in bacterial vectors.
In the practical exercises the student will apply these techniques for purification of plasmid DNA and genomic DNA, separation of DNA by electrophoresis on agarose gel, its digestion with restriction enzymes, preparation of gene constructs and their transformation into E. coli.
At the end of the course, the student will have learned the basics of manipulation of genetic material and will be able to use the main techniques for gene cloning.


The recombinant DNA. Cloning of Genes.
Bacterial and non bacterial vectors. cDNA and genomic libraries.
Manipulation of nucleic acids. Digestion with restriction endonucleases. Electrophoresis. Ligation of the DNA.
Polymerase Chain Reaction (PCR) and its applications.
Bacteria colutres. Trasformation of bacterial and eukaryotic cells.
Production of recombinant proteins.

Laboratory experiences:
Purification of plasmid DNA
Purification of genomic DNA
Purification of RNA
Electrophoresis and purification of DNA from gel.
Digestion with restriction enzymes.
PCR and PCR product purification.
Transformation, colony PCR
Expression of a recombinant protein and its analysis by SDS PAGE and Western Blot.

Assessment methods and criteria

Laboratory activities report and oral examination

Reference books
Activity Author Title Publisher Year ISBN Note
Teoria Karcher LABORATORIO DI BIOLOGIA MOLECOLARE ed Zanichelli 1998
Teoria Michael R. Green e Joseph Sambrook Molecular Cloning: A Laboratory Manual, Fourth Edition (Edizione 4) CSHL Press 2012 978-1-936113-42-2
Laboratorio Amaldi, Benedetti, Pesole, Plevani Biologia Molecolare (Edizione 3) Ambrosiana 2018 978-88-08-18518-1