|Teoria||2||I sem.||Stefano Capaldi|
|Laboratorio [II turno]||4||I sem.||Maria Teresa Valenti|
|Laboratorio [I turno]||4||I sem.||Stefano Capaldi|
|Teoria||Thursday||1:30 PM - 3:30 PM||lesson||Lecture Hall B|
|Laboratorio [I turno]||Friday||9:30 AM - 1:30 PM||laboratorio||Laboratory Laboratorio di Biotecnologie genetiche||from Oct 28, 2016 to Nov 11, 2016|
|Laboratorio [I turno]||Friday||2:30 PM - 6:30 PM||laboratorio||Laboratory Laboratorio di Biotecnologie genetiche||from Oct 28, 2016 to Nov 11, 2016|
|Laboratorio [II turno]||Friday||10:30 AM - 6:30 PM||laboratorio||Laboratory Laboratorio di Biotecnologie genetiche||from Nov 18, 2016 to Jan 31, 2017|
The primary educational aim of the course is to provide the student an overview on the most common methodologies and techniques for the manipulation of DNA. In particular, the course covers the main techniques for purification of nucleic acids, their separation by electrophoresis, DNA amplification and methods for cloning in bacterial vectors.
In the practical exercises the student will apply these techniques for purification of plasmid DNA and genomic DNA, separation of DNA by electrophoresis on agarose gel, its digestion with restriction enzymes, preparation of gene constructs and their transformation into E. coli.
At the end of the course, the student will have learned the basics of manipulation of genetic material and will be able to use the main techniques for gene cloning.
The recombinant DNA. Cloning of Genes.
Bacterial and non bacterial vectors. cDNA and genomic libraries.
Manipulation of nucleic acids. Digestion with restriction endonucleases. Electrophoresis. Ligation of the DNA.
Polymerase Chain Reaction (PCR) and its applications.
Bacteria colutres. Trasformation of bacterial and eukaryotic cells.
Production of recombinant proteins.
Purification of plasmid DNA
Purification of genomic DNA
Purification of RNA
Electrophoresis and purification of DNA from gel.
Digestion with restriction enzymes.
PCR and PCR product purification.
Transformation, colony PCR
Expression of a recombinant protein and its analysis by SDS PAGE and Western Blot.
Laboratory activities report and oral examination
|Teoria||Karcher||LABORATORIO DI BIOLOGIA MOLECOLARE||ed Zanichelli||1998|
|Teoria||Michael R. Green e Joseph Sambrook||Molecular Cloning: A Laboratory Manual, Fourth Edition (Edizione 4)||CSHL Press||2012||978-1-936113-42-2|
|Laboratorio||Amaldi, Benedetti, Pesole, Plevani||Biologia Molecolare (Edizione 3)||Ambrosiana||2018||978-88-08-18518-1|